Spinning disk confocal psf8/31/2023 ![]() In order to assure high image acquisition speeds of fluorescent proteins and synthetic dyes in live cells with reasonable contrast and minimal photobleaching, microscopes must be able to quickly scan the field of view and record data using detectors with high quantum efficiency. Acquiring images of localized fluorophores in living cells on the millisecond timescales that reveal intricate biological dynamics presents a host of new challenges, which are far more complex than the traditional issues associated with creating a single high-resolution snapshot of well-stained fixed tissue in a laser scanning confocal microscope. ![]() The explosive growth in biomedical research using live-cell imaging techniques has been fueled by a combination of events that include dramatic advances in confocal microscopy instrumentation coupled with the introduction of novel ultra-sensitive detectors and continued improvements in the performance of genetically-encoded fluorescent proteins. ![]()
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